Romanosky A J, Bagby G J, Bockman E L, Spitzer J J
Am J Physiol. 1980 Dec;239(6):E391-5. doi: 10.1152/ajpendo.1980.239.6.E391.
The effect of endotoxin administration on free fatty acid (FFA) utilization by skeletal muscle was investigated. Albumin bound [1-14C]palmitate was continuously infused into anesthetized dogs. Blood samples from the carotid artery and profunda femoris veins were obtained and blood flow through the thigh muscles was determined. After endotoxin, skeletal muscle blood flow decreased by 29%, reflecting the average decrease in cardiac output. Arterial FFA concentration in endotoxin-treated animals was significantly reduced relative to saline-treated animals. Whole-animal FFA turnover decreased up to 37% after endotoxin. FFA uptake was linearly correlated with arterial FFA concentration both preceding and following endotoxin and the slopes of the regression lines were identical. FFA uptake by skeletal muscle was significantly reduced during the early postendotoxin time period. Skeletal muscle FFA oxidation after endotoxin also tended to decrease. It is concluded that the changes in skeletal muscle FFA metabolism are most likely due to decreased arterial FFA levels after endotoxin rather than a direct effect of endotoxin on skeletal muscle.
研究了内毒素给药对骨骼肌游离脂肪酸（FFA）利用的影响。将白蛋白结合的[1-14C]棕榈酸连续输注到麻醉犬体内。采集颈动脉和股深静脉血样，并测定流经大腿肌肉的血流量。给予内毒素后，骨骼肌血流量下降了29%，反映出心输出量的平均下降。与生理盐水处理的动物相比，内毒素处理动物的动脉FFA浓度显著降低。内毒素给药后，全动物FFA周转率下降高达37%。内毒素给药前后，FFA摄取与动脉FFA浓度呈线性相关，回归线斜率相同。内毒素给药后的早期，骨骼肌对FFA的摄取显著减少。内毒素给药后，骨骼肌FFA氧化也有下降趋势。结论是，骨骼肌FFA代谢的变化很可能是由于内毒素给药后动脉FFA水平降低，而非内毒素对骨骼肌的直接作用。
