Lew A M, Hosking C S, Studdert M J
Am J Vet Res. 1980 Aug;41(8):1161-6.
Tests for T- and B-cell quantitation and immune function were developed, and their application in the diagnosis of primary severe combined immunodeficiency disease (CID) in Arabian foals was investigated. Foals with CID had severe lymphopenia and had small or zero numbers of B cells, as shown by immunofluorescence of surface immunoglobulin (Ig), erythrocyte-antibody-complement rosetting, and staphylococcal protein A rosetting. Serum IgM was undetectable in four CID foals 25 to 71 days old. Demonstrable antibody responses were not elicited in CID foals by phage phi X-174, a potent antigen in normal foals. Nonspecific esterase (NSE) staining in the pattern of a single vesicle was investigated as a possible marker for equine T cells. For normal foals, 64.0% of peripheral blood lymphocytes stained NSE positive. The CID foal 1 had only 4.0% NSE-positive lymphocytes, whereas CID foals 2, 3, and 4 had 75%, 68%, and 77.5%, respectively. In an in vitro T-cell function test, lymphocytes from 12 normal foals did not show a response. In normal foals, intradermal injection of 50 micrograms of phytohemagglutinin induced visible reactions, and skin grafting induced a pronounced mononuclear cell response at the base of the graft. In contrast, there was little or no response in the foals with CID.
开发了用于T细胞和B细胞定量及免疫功能检测的方法，并研究了它们在诊断阿拉伯马驹原发性严重联合免疫缺陷病（CID）中的应用。患有CID的马驹出现严重淋巴细胞减少，且B细胞数量很少或为零，这通过表面免疫球蛋白（Ig）免疫荧光、红细胞 - 抗体 - 补体花环试验和葡萄球菌蛋白A花环试验得以证实。在4匹25至71日龄的CID马驹中未检测到血清IgM。噬菌体φX - 174（正常马驹中的一种强抗原）未能在CID马驹中引发可证实的抗体反应。研究了单个囊泡模式的非特异性酯酶（NSE）染色作为马T细胞可能标志物的情况。对于正常马驹，64.0%的外周血淋巴细胞NSE染色呈阳性。CID马驹1仅有4.0%的NSE阳性淋巴细胞，而CID马驹2、3和马驹4分别为75%、68%和77.5%。在一项体外T细胞功能试验中，12匹正常马驹的淋巴细胞未显示出反应。在正常马驹中，皮内注射50微克植物血凝素会引发可见反应，皮肤移植会在移植部位底部引发明显的单核细胞反应。相比之下，患有CID的马驹反应很少或没有反应。
