心肌溶酶体酶对分离线粒体的蛋白水解作用。
Proteolysis of isolated mitochondria by myocardial lysosomal enzymes.
作者信息
Allan R M, Welman E
出版信息
Biochem J. 1980 Jul 15;190(1):139-44. doi: 10.1042/bj1900139.
Abstract
- Solubilized mitochondria and lysosomal fractions were obtained from guinea-pig heart by differential centrifugation and selective membrane disruption. 2. Mitochondria incubated at 37 degrees C in the presence of lysosomal enzymes underwent proteolysis. The rate of protein degradation was inversely dependent on pH. 3. The use of proteinase inhibitors showed that at low pH the major enzyme involved in mitochondrial digestion was cathepsin D. 4. At neutral pH carboxyl proteinases were still active, but thiol proteinases accounted for most of the protein breakdown. 5. The role of lysosomal enzymes as mediators of mitochondrial damage in ischaemic myocardium is discussed.
摘要
- 通过差速离心和选择性膜破坏从豚鼠心脏中获得溶解的线粒体和溶酶体组分。2. 在溶酶体酶存在下于37℃孵育的线粒体发生了蛋白水解。蛋白质降解速率与pH呈负相关。3. 使用蛋白酶抑制剂表明，在低pH值下，参与线粒体消化的主要酶是组织蛋白酶D。4. 在中性pH值下，羧基蛋白酶仍然活跃，但巯基蛋白酶占蛋白质分解的大部分。5. 讨论了溶酶体酶作为缺血心肌中线粒体损伤介质的作用。
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