在离体灌注大鼠肾脏中前列腺素F2α向前列腺素E2的肾内转化及肾素释放

Intrarenal conversion of prostaglandin F2 alpha into prostaglandin E2 and renin release in the isolated perfused rat kidney.

Schwertschlag U, Seyberth H W, Müller H, Grunewald R, Erlenmaier T, Rohs H G, Hackenthal E

Clin Sci (Lond). 1980 Dec;59 Suppl 6:117s-119s. doi: 10.1042/cs059117s.

Abstract

Isolated rat kidneys were perfused with a modified Krebs-Henseleit medium at constant pressure. 2. When prostaglandin F2 alpha (PGF2 alpha) was infused into these kidneys at 0.1 mumol/l (final concentration) PGF2 alpha-derived prostaglandin E2 (PGE2) could be identified by high-performance liquid chromatography and combined gas chromatography-mass spectrometry. 3. The extent of this conversion of PGF2 alpha into PGE2 during passage through the kidney is dependent on the salt history of the rats from which the kidneys were taken for perfusion: kidneys from rats kept on normal diet converted 10%, those from rats on a low sodium diet 5% and those from rats kept on a high sodium diet 11%. 4. These differences in conversion can account for the different increases in renin release after PGF2 alpha infusion in these groups.

摘要

将离体大鼠肾脏在恒定压力下用改良的克雷布斯 - 亨泽莱特培养基灌注。2. 当以0.1微摩尔/升（终浓度）将前列腺素F2α（PGF2α）注入这些肾脏时，可通过高效液相色谱和气相色谱 - 质谱联用技术鉴定出PGF2α衍生的前列腺素E2（PGE2）。3. 在肾脏灌注过程中，PGF2α转化为PGE2的程度取决于用于灌注的大鼠的盐摄入史：取自正常饮食大鼠的肾脏转化率为10%，低钠饮食大鼠的肾脏转化率为5%，高钠饮食大鼠的肾脏转化率为11%。4. 这些转化率的差异可以解释这些组中PGF2α注入后肾素释放增加的不同情况。