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禁食大鼠消化过程中肝细胞线粒体的体视学分析。

Stereologic analysis of mitochondria of hepatocyte from fasted rats in the course of digestion.

作者信息

Cieciura L, Klitończyk W, Rydzyński K

出版信息

Folia Histochem Cytochem (Krakow). 1978;16(3):193-203.

PMID:700506
Abstract

Changes of the configurational states of the rat's hepatocyte mitochondria after 18 hours fasting and then one, two, three, four hours after feeding were investigated with stereological methods. Among others morphometric parameters coefficient of share (E) was introduced to express external or internal mitochondrial compartment volume per unit of surface area of the internal membrane. For parameters variation analysis Kruskal-Wallis test was used. Application of stereological methods reveals subtle changes lying beyond the power of visual observation and make possible the recognition of ultrastructural configuration of mitochondria in hepatocyte in the course of fasting and digestion connected with the various metabolic state.

摘要

采用体视学方法研究了大鼠肝细胞线粒体构型状态在禁食18小时后以及喂食后1、2、3、4小时的变化。除其他形态测量参数外,引入了份额系数(E)来表示内膜单位表面积的线粒体外腔或内腔体积。对于参数变化分析,使用了Kruskal-Wallis检验。体视学方法的应用揭示了肉眼观察无法察觉的细微变化,并使得在禁食和消化过程中与各种代谢状态相关的肝细胞线粒体超微结构构型得以识别。

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