Friedberg S J, Gomillion M
J Biol Chem. 1981 Jan 10;256(1):291-5.
We have previously presented evidence that acyl dihydroxyacetone phosphate is converted to O-alkyl dihydroxyacetone phosphate via an endiol intermediate which then accepts a fatty alcohol to form O-alkyl dihydroxyacetone phosphate. We have further proposed that, in the absence of fatty alcohol, the endiol derivative of acyl dihydroxyacetone phosphate reacts with water to form dihydroxyacetone phosphate. In support of this hypothesis, we have shown, in an O-alkyl generating system, that the amount of hydrogen released from acyl dihydroxyacetone phosphate in the formation of the endiol is greater than the amount of hydrogen lost from the total lipid present at the end of incubation. The discrepancy is greater in the absence of added hexadecanol. The balance of the hydrogen loss can be accounted for by the formation of a non-lipid substance which was identified as dihydroxyacetone phosphate.
我们之前已提供证据表明，酰基二羟基丙酮磷酸酯通过烯二醇中间体转化为O - 烷基二羟基丙酮磷酸酯，该烯二醇中间体随后接受脂肪醇形成O - 烷基二羟基丙酮磷酸酯。我们进一步提出，在没有脂肪醇的情况下，酰基二羟基丙酮磷酸酯的烯二醇衍生物与水反应形成二羟基丙酮磷酸酯。为支持这一假设，我们在一个O - 烷基生成系统中表明，在形成烯二醇的过程中，酰基二羟基丙酮磷酸酯释放的氢气量大于孵育结束时存在的总脂质损失的氢气量。在没有添加十六醇的情况下，这种差异更大。氢气损失的平衡可以通过形成一种非脂质物质来解释，该物质被鉴定为二羟基丙酮磷酸酯。
