Franchimont C, Pierard G E
J Cutan Pathol. 1980 Dec;7(6):387-93. doi: 10.1111/j.1600-0560.1980.tb01212.x.
A histopathologic study of side effects of chemotherapy on normal skin was made by light microscopy and radioautography after incorporation of tritiated thymidine. Two psoriatic patients were studied after methotrexate treatment and one after polychemotherapy for an osteosarcoma 2--5 days after administration of methotrexate. Four biological effects can occur: cellular death, recruitment of cells from a resting phase into the cell cycle of proliferation, synchronization of cells in that cycle and blockage of cells in specific phases. Sub-lethal signs and necrosis were recognized in the epidermis as vacuolar alterations, cytoplasmic inclusions, swelling of keratinocytes, apoptosis and dyskeratosis. Bullae resulted from epidermal necrolysis. The labelling index of normal looking keratinocytes was very high. Within capillaries, plump and hyperchromatic endothelial cells were increased in number, as was their labelling index. Fibroblasts and cells of the eccrine glands were also labelled in number. The number of labelled nuclei within these cell lines reflects the recruitment and synchronization in the cell cycle and the blockage in S phase.
在掺入氚标记的胸腺嘧啶核苷后，通过光学显微镜和放射自显影术对化疗对正常皮肤的副作用进行了组织病理学研究。对两名银屑病患者在接受甲氨蝶呤治疗后进行了研究，对一名骨肉瘤患者在给予甲氨蝶呤后2 - 5天进行多药化疗后进行了研究。可出现四种生物学效应：细胞死亡、将处于静止期的细胞募集到增殖细胞周期中、使细胞在该周期中同步以及使细胞在特定阶段受阻。在表皮中，亚致死体征和坏死表现为空泡样改变、胞质内含物、角质形成细胞肿胀、凋亡和角化异常。大疱是由表皮坏死松解引起的。外观正常的角质形成细胞的标记指数非常高。在毛细血管内，丰满且核染色质增多的内皮细胞数量增加，其标记指数也增加。成纤维细胞和汗腺细胞的数量也有标记。这些细胞系中标记核的数量反映了细胞周期中的募集和同步以及S期的阻滞。
