一种用于检测脑脊液中GM1神经节苷脂浓度的放射免疫分析方法。

A radioassay for GM1 ganglioside concentration in cerebrospinal fluid.

作者信息

Ginns E, French J

出版信息

J Neurochem. 1980 Oct;35(4):977-82. doi: 10.1111/j.1471-4159.1980.tb07097.x.

DOI:10.1111/j.1471-4159.1980.tb07097.x

Abstract

A radioassay for the rapid determination of GM1 ganglioside concentration in small volumes of CSF from individual patients is described. The assay utilizes the high-affinity interaction between cholera enterotoxin and GM1 ganglioside. The lower detection limit of GM1 ganglioside by this radioassay under the described incubation conditions is 2.5 ng/ml. The radioassay-determined lumbar CSF GM1 ganglioside concentrations in a small group of patients with diverse neurologic disorders are presented. The radioassay GM1 ganglioside concentration is in good agreement with the GM1 ganglioside concentration determined, in one patient, by the tlc-densitometry technique.

摘要

本文描述了一种用于快速测定个体患者少量脑脊液中GM1神经节苷脂浓度的放射免疫测定法。该测定法利用霍乱肠毒素与GM1神经节苷脂之间的高亲和力相互作用。在所描述的孵育条件下，该放射免疫测定法对GM1神经节苷脂的检测下限为2.5 ng/ml。文中给出了一小群患有各种神经系统疾病患者的放射免疫测定法测定的腰椎脑脊液GM1神经节苷脂浓度。在一名患者中，放射免疫测定法测定的GM1神经节苷脂浓度与薄层层析光密度法测定的GM1神经节苷脂浓度高度一致。