Schöch G
Fortschr Med. 1978 Oct 5;96(37):1875-80.
All known nucleic acids contain varying amounts of modified nucleosides. In contrast to the standard nucleosides, the modified nucleosides and their nucleobases as minor nucleic acids components cannot be recycled. Only part of them can be catabolized. Therefore, many modified catabolites of nucleic acids are enriched in urine. They can be measured at the picomole level by cation exchange HPLC. Plotted against age, the excretion curves of the modified nucleobases are in accordance with the shape of the known growth velocity curve. Aberrant nucleobases excretion patterns are found in very young children, probably resulting from a specific nucleic acids equipment as well as from different relative growth velocities of different organs. In the urine of patients with neoplasms, often extremely disturbed nucleobases excretion patterns are found which can be normalized by successful cytostatic therapy. From the published and our own results it is concluded that modifications of nucleic acids should play an important regulatory role. Therefore, the analysis of modified nucleobases excretion patterns will open new diagnostic approaches.
所有已知的核酸都含有不同数量的修饰核苷。与标准核苷不同，修饰核苷及其核碱基作为次要的核酸成分不能被循环利用。只有一部分可以被分解代谢。因此，许多核酸的修饰分解代谢产物在尿液中富集。它们可以通过阳离子交换高效液相色谱法在皮摩尔水平上进行测量。将修饰核碱基的排泄曲线与年龄作图，其形状与已知的生长速度曲线一致。在非常年幼的儿童中发现异常的核碱基排泄模式，这可能是由于特定的核酸组成以及不同器官不同的相对生长速度所致。在肿瘤患者的尿液中，经常发现极其紊乱的核碱基排泄模式，而成功的细胞抑制疗法可使其恢复正常。根据已发表的和我们自己的结果可以得出结论，核酸修饰应发挥重要的调节作用。因此，对修饰核碱基排泄模式的分析将开辟新的诊断途径。
