Martin S M, Glick B R, Martin W G
Can J Microbiol. 1980 Oct;26(10):1209-13. doi: 10.1139/m80-202.
An examination of conditions for the growth of Desulfovibrio desulfuricans, with the aim of optimizing hydrogenase production, is reported. An ammonium sulfate-lactate-yeast extract medium gave 5 to 10 times as much hydrogenase activity as a peptone-yeast extract medium. It made little if any difference whether the gas used for sparging was nitrogen, hydrogen, or a mixture thereof but increasing the rate of sparging and agitation did result in a slight decrease in activity. Control of pH during culture development was of little benefit to hydrogenase production. At least two hydrogenases were present in D. desulfuricans: one periplasmic, the other membrane bound.
报道了一项针对脱硫脱硫弧菌生长条件的研究，目的是优化氢化酶的产量。硫酸铵-乳酸-酵母提取物培养基产生的氢化酶活性是蛋白胨-酵母提取物培养基的5至10倍。用于鼓泡的气体是氮气、氢气还是它们的混合物，几乎没有差别，但提高鼓泡速率和搅拌速率确实会导致活性略有下降。在培养过程中控制pH值对氢化酶的产生几乎没有益处。脱硫脱硫弧菌中至少存在两种氢化酶：一种存在于周质中，另一种与膜结合。
