Pelliniemi L J, Dym M, Gunsalus G L, Musto N A, Bardin C W, Fawcett D W
Endocrinology. 1981 Mar;108(3):925-31. doi: 10.1210/endo-108-3-925.
The localization of androgen-binding protein (ABP) in the reproductive tract of young adult male rats was studied with the peroxidase-antiperoxidase technique using frozen sections and light microscopy. Within the seminiferous tubules, a positive reaction was noted in the apical portion of the epithelium, apparently in spermatids and/or Sertoli cells. ABP was localized in granules in the apical cytoplasm of the principal epithelial cells of the proximal part of the caput epididymis and in the epithelial cells of the ductuli efferentes. The cells in the distal part of the caput as well as the corpus and cauda of the epididymis did not contain ABP. Numerous coated vesicles and multivesicular bodies were present in the supranuclear cytoplasm of the epididymal epithelium where ABP was taken up. The results indicate that ABP is taken up from the lumen by epithelial cells of the ductuli efferentes and proximal part of the caput epididymis.
采用过氧化物酶-抗过氧化物酶技术，利用冰冻切片和光学显微镜，研究了雄激素结合蛋白（ABP）在成年雄性幼鼠生殖道中的定位。在生精小管内，上皮顶端部分出现阳性反应，显然是在精子细胞和/或支持细胞中。ABP定位于附睾头近端主上皮细胞顶端细胞质的颗粒中以及输出小管的上皮细胞中。附睾头远端以及附睾体和尾的细胞不含ABP。在附睾上皮超核细胞质中存在大量有被小泡和多泡体，ABP在此被摄取。结果表明，ABP是由输出小管和附睾头近端的上皮细胞从管腔中摄取的。
