Jezek D, Simunić-Banek L, Pezerović-Panijan R
Institute of Histology and Embryology, Medical Faculty University of Zagreb, Croatia.
Arch Toxicol. 1993;67(2):131-40. doi: 10.1007/BF01973684.
The effects of exogenous testosterone on various testicular variables has become of increasing significance because of its potential use in male contraception. For this reason, high doses of two testosterone esters [testosterone propionate (TP) and testosterone enanthate (TE)] were used in a study of their influence on the morphology, length and curvature of the seminiferous tubules of the rat testis, and on cytological smears of the seminiferous tubules epithelium. TP was given for 14 days (3 mg/100 g body weight, i.m.) to assess the acute effects of testosterone on the seminiferous tubules. TE was administered for 60 days (in the same manner as TP) to study possible chronic effects on the rat testis. After TP and TE treatment the seminiferous tubule epithelium showed disorganization and desquamation of spermatogenic cells. In the TP-treated testes the tubules lined with Sertoli cells only were observed. The values for the length and curvature of seminiferous tubules of the TP- and TE-treated rats were significantly reduced (p < 0.001). All these changes were observed earlier in the TP-treated than in the TE-treated animals. In cytological smears of the testis of the TP- and TE-treated rats an increase of vacuoles and residual bodies in Sertoli cell cytoplasm was noted. In addition, a reduction of spermatogenic cells, particularly sperms, was manifest in the smears after treatment. Large groups of Sertoli cells were seen in the smears from these testes.
由于外源性睾酮在男性避孕方面的潜在用途，其对各种睾丸变量的影响变得越来越重要。因此，在一项研究中使用了高剂量的两种睾酮酯[丙酸睾酮（TP）和庚酸睾酮（TE）]，以研究它们对大鼠睾丸生精小管的形态、长度和曲率以及生精小管上皮细胞涂片的影响。给予TP 14天（3mg/100g体重，肌肉注射）以评估睾酮对生精小管的急性影响。给予TE 60天（方式与TP相同）以研究对大鼠睾丸可能的慢性影响。TP和TE处理后，生精小管上皮显示出生精细胞的紊乱和脱落。在TP处理的睾丸中，仅观察到由支持细胞内衬的小管。TP和TE处理的大鼠生精小管的长度和曲率值显著降低（p<0.001）。所有这些变化在TP处理的动物中比在TE处理的动物中更早观察到。在TP和TE处理的大鼠睾丸的细胞学涂片中，观察到支持细胞胞质中的空泡和残余小体增加。此外，处理后的涂片中可见生精细胞，尤其是精子数量减少。在这些睾丸的涂片中可见大量支持细胞。
