Bresler S E, Verbenko V N, Kalinin V L
Genetika. 1980;16(10):1753-63.
The strains of Bacillus subtilis actively exchange genetic material during joint incubation on a properly composed selective solid medium at 37 degrees C. Predominantly, colonies of multiple recombinants are formed. If DNase is present in the medium, total number of recombinant colonies and the share of colonies of multiple recombinants decrease. At 46 degrees C the recombination process is inhibited. The results obtained are discussed using two hypotheses: 1) a donor cell transfers a significant part or even whole chromosome to a neighbouring recipient cell, 2) recombinations occur successively in mini-clones of intermediate recombinants. It is suggested that the phenomenon observed is close to the natural genetic transformation in Bac. subtilis.
枯草芽孢杆菌菌株在37摄氏度的适当组成的选择性固体培养基上共同培养期间会积极交换遗传物质。主要形成多个重组体的菌落。如果培养基中存在DNA酶,重组菌落的总数和多个重组体菌落的比例会降低。在46摄氏度时,重组过程受到抑制。使用两个假说来讨论所获得的结果:1)供体细胞将很大一部分甚至整个染色体转移到相邻的受体细胞,2)在中间重组体的小克隆中连续发生重组。有人认为所观察到的现象与枯草芽孢杆菌中的自然遗传转化相近。