Senior B W
J Med Microbiol. 1981 Feb;14(1):151-2. doi: 10.1099/00222615-14-1-151.
A rapid and simple method is described by which colonies of Proteus can be distinguished from those of Salmonella and Shigella and other non-lactose-fermenting organisms growing on MacConkey's agar or desoxycholate citrate agar. The method is based on the ability of Proteus to produce urease constitutively. The enzyme was detected by the degradation of urea by the inoculum, thereby creating an alkaline reaction on pH-indicator paper.
本文描述了一种快速简便的方法，可用于区分变形杆菌菌落与沙门氏菌、志贺氏菌以及在麦康凯琼脂或脱氧胆酸盐柠檬酸盐琼脂上生长的其他非乳糖发酵菌的菌落。该方法基于变形杆菌组成型产生脲酶的能力。通过接种物对尿素的降解来检测该酶，从而在pH指示纸上产生碱性反应。
