Nerurkar S G, Gambhir K K
Clin Chem. 1981 Apr;27(4):607-9.
In vitro hemolysates of isolated human erythrocytes degrade 125I-labeled insulin. Ten- to 100-fold dilutions of the hemolysate give a proportionately decreased degradation of 125I-labeled insulin at 37 degrees C, while dilutions of up to eightfold do not. Like the control, diluted "Buffer G" containing 5 mmol/L Tris and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer alone, more than 500-fold dilutions of the hemolysate or boiled hemolysate (in buffer) caused negligible (less than 1%) degradation of the labeled insulin. We conclude that accurate insulin-binding data during erythrocyte insulin radioreceptor assay under optimum conditions (Clin. Chem. 23: 1590-1595, 1977) depend on avoiding or minimizing hemolysis.
分离的人红细胞的体外溶血产物可降解¹²⁵I标记的胰岛素。溶血产物稀释10至100倍时，在37℃下¹²⁵I标记的胰岛素降解程度相应降低，而稀释至八倍时则不会。与对照一样，仅含5 mmol/L Tris和4-（2-羟乙基）-1-哌嗪乙磺酸缓冲液的稀释“缓冲液G”、溶血产物稀释500倍以上或煮沸的溶血产物（在缓冲液中）对标记胰岛素的降解可忽略不计（小于1%）。我们得出结论，在最佳条件下进行红细胞胰岛素放射受体测定时（临床化学23: 1590 - 1595, 1977），准确的胰岛素结合数据取决于避免或尽量减少溶血。
