Laurent F, Gross R, Lakili M, Mialhe P
Diabetologia. 1981;20(1):72-7. doi: 10.1007/BF00253821.
A possible action of insulin via glucose metabolism on the pancreatic A cell response to glucose, was studied in ducks. 2-Deoxyglucose, a nonmetabolizable analogue of glucose was used. In normal ducks, the hyperglycaemia induced by 2-deoxyglucose (IV: 0.5 g/kg) resulted in hyperglucagonaemia, while the same degree of hyperglycaemia, induced by glucose infusion (IV injection 25 mg/kg, and infusion 5 mg/kg/min) immediately suppressed glucagon secretion. In diabetic ducks, two days after subtotal pancreatectomy, glucose responsiveness of the A cell was abolished, but could be restored by insulin treatment before (IM 0.2 U/kg insulin + 8 micrograms/kg glucagon every 6 h) and during (IV 3.6 mU/kg + infusion 0.9 mU/kg/min) the glucose test (IV: 0.5 g/kg). The normal response of the A cell to glucose was not observed in diabetic insulin-treated ducks after the administration of 2-deoxyglucose (IV: 0.5 g/kg). These data suggest an inhibitory effect of the metabolism of glucose on the release of glucagon. In addition, the action of insulin on the A cell may be mediated by its effect on glucose metabolism within the A cell.
在鸭身上研究了胰岛素通过葡萄糖代谢对胰腺 A 细胞对葡萄糖反应的可能作用。使用了葡萄糖的非代谢类似物 2-脱氧葡萄糖。在正常鸭中，2-脱氧葡萄糖（静脉注射：0.5 g/kg）诱导的高血糖导致高胰高血糖素血症，而葡萄糖输注（静脉注射 25 mg/kg，输注 5 mg/kg/min）诱导的相同程度的高血糖立即抑制胰高血糖素分泌。在胰腺次全切除术后两天的糖尿病鸭中，A 细胞的葡萄糖反应性消失，但在葡萄糖试验（静脉注射：0.5 g/kg）前（肌肉注射 0.2 U/kg 胰岛素 + 每 6 小时 8 μg/kg 胰高血糖素）和期间（静脉注射 3.6 mU/kg + 输注 0.9 mU/kg/min）给予胰岛素治疗可恢复。给予 2-脱氧葡萄糖（静脉注射：0.5 g/kg）后，在糖尿病胰岛素治疗的鸭中未观察到 A 细胞对葡萄糖的正常反应。这些数据表明葡萄糖代谢对胰高血糖素释放有抑制作用。此外，胰岛素对 A 细胞的作用可能是通过其对 A 细胞内葡萄糖代谢的影响来介导的。
