Anderton B H, Thorpe R, Cohen J, Selvendran S, Woodhams P
J Neurocytol. 1980 Dec;9(6):835-44. doi: 10.1007/BF01205022.
Rabbit antisera have been produced to bovine brain 10 nm filament preparations and to purified polypeptide constituents of brain 10 nm filaments. Antisera to the former preparation stain both neurons and astrocytes, whereas antisera to two polypeptides of mol. wt 155 000 and 210 000 are neuron specific. It is therefore concluded that these latter polypeptides are components of neurofilaments and the mixed staining pattern obtained with the antisera to the whole brain 10 nm filament preparation is due to the presence in this material of a mixture of neurofilaments and glial filaments which are thus biochemically distinct forms of 10 nm filament.
已制备出针对牛脑10纳米细丝制剂以及脑10纳米细丝纯化多肽成分的兔抗血清。针对前一种制剂的抗血清可使神经元和星形胶质细胞均显色，而针对分子量为155000和210000的两种多肽的抗血清则具有神经元特异性。因此可以得出结论，后一种多肽是神经丝的组成成分，用针对全脑10纳米细丝制剂的抗血清所获得的混合染色模式是由于该材料中存在神经丝和胶质丝的混合物，因此它们是10纳米细丝在生化上的不同形式。
