Petkov M
Vet Med Nauki. 1980;17(4):3-9.
Through the implementation of the technique of ligated intestinal segments with established the enteropathogenic character of strains E. coli, isolated from calves by means of enteritis. The enteropathogenic E. coli provoked dilatation of the ligated intestinal segments on the basis of the enterotoxin produced by them, whereas in the case of the non-enteropathogenic ones such a reaction was not observed. The dilated intestinal segments were filled with an exudate of watery consistency under pressure and an edema of the mucosis and mesentery was observed. The strain investigated is determined as enteropathogenic or not if it causes dilatation of the ligated intestinal segment in two of the calves tested. Out of the 272 E. coli strains only 43/15.8% proved to be enteropathogenic. The enteropathogenic E. coli refer most frequently to serotypes 08,0101 and 09.
通过采用结扎肠段技术，确定了从患肠炎的犊牛中分离出的大肠杆菌菌株的肠道致病特性。致病性大肠杆菌基于其产生的肠毒素导致结扎肠段扩张，而非致病性大肠杆菌则未观察到这种反应。扩张的肠段在压力下充满了水样渗出物，且观察到黏膜和肠系膜水肿。如果所研究的菌株在两只受试犊牛中引起结扎肠段扩张，则可确定其为致病性或非致病性。在272株大肠杆菌菌株中，只有43株（15.8%）被证明具有致病性。致病性大肠杆菌最常见的血清型为O8、O101和O9。
