[Demonstration of enteropathogenic E. coli by the ligated intestinal segment technic].

Through the implementation of the technique of ligated intestinal segments with established the enteropathogenic character of strains E. coli, isolated from calves by means of enteritis. The enteropathogenic E. coli provoked dilatation of the ligated intestinal segments on the basis of the enterotoxin produced by them, whereas in the case of the non-enteropathogenic ones such a reaction was not observed. The dilated intestinal segments were filled with an exudate of watery consistency under pressure and an edema of the mucosis and mesentery was observed. The strain investigated is determined as enteropathogenic or not if it causes dilatation of the ligated intestinal segment in two of the calves tested. Out of the 272 E. coli strains only 43/15.8% proved to be enteropathogenic. The enteropathogenic E. coli refer most frequently to serotypes 08,0101 and 09.