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[高效价支气管败血波氏杆菌凝集血清的制备]

[Production of a high-titer agglutinating Bordetella bronchiseptica serum].

作者信息

Ganovski D, Mermerski K

出版信息

Vet Med Nauki. 1980;17(4):50-2.

PMID:7010770
Abstract

For a serological identification of Bordetella Bronchiseptica, eliminated from swine ill with atrophic rhinitis, was obtained an agglutinated antiserum. For the purpose rabbits were innoculated 1. w. 2.5-3 kg) and living cultures were used after intravenous injection. For the obtention of a bacterial suspension Müller-Hinton's medium was used. Three series of antiserum were produced against Bordetella Bronchiseptica. The first series was obtained after straining the bacterial strain into the medium of McGengou and Müller-Hinton agar and had a high titre 1:640-:1280. The second series was obtained after straining the strain through egg embryos and had a titre 1:5000-:10,000. By straining the strain through egg embryos and young white mice, an antiserum was obtained having the titre 1:20,000-1:40,000.

摘要

为了对从患有萎缩性鼻炎的猪身上分离出的支气管败血波氏杆菌进行血清学鉴定,制备了凝集抗血清。为此,选用体重2.5 - 3千克的家兔进行静脉注射,使用活培养物。为获得细菌悬液,使用了米勒-欣顿培养基。制备了针对支气管败血波氏杆菌的三批抗血清。第一批抗血清是将菌株接种于麦康凯培养基和米勒-欣顿琼脂培养基后获得的,效价为1:640 - 1:1280。第二批抗血清是将菌株通过鸡胚传代后获得的,效价为1:5000 - 1:10000。通过将菌株通过鸡胚和幼小白鼠传代,获得了效价为1:20000 - 1:40000的抗血清。

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