Rosen O M, Rubin C S, Cobb M H, Smith C J
J Biol Chem. 1981 Apr 25;256(8):3630-3.
Physiological concentrations of insulin stimulate the incorporation of 32P into ribosomal protein S6 in intact 3T3-L1 adipocytes (Smith, C. J., Wejksnora, P. J., Warner, J. B., Rubin, C. S., and Rosen, O. M. (1979) Proc. Natl. Acad. Sci. U. S. A. 76, 2725-2729). Extracts from cells treated with insulin also exhibit an increased ability to incorporate 32P from [gamma-32P]ATP into protein S6 in vitro (Smith, C. J., Rubin, C. S., and Rosen, O. M. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 2641-2645). We now report that the direct addition of insulin to particulate preparations containing high affinity insulin receptors and ribosomes derived from insulin-sensitive cells (not previously exposed to insulin) stimulates incorporation of 32P from [gamma-32P]ATP into ribosomal protein S6 1.5- to 3.0-fold. The stimulation occurs at the same concentrations of insulin (0.1-1.0 nM) and within the same period of time as it does in intact cells.
生理浓度的胰岛素可刺激完整的3T3 - L1脂肪细胞将³²P掺入核糖体蛋白S6中（史密斯，C.J.，韦克斯诺拉，P.J.，华纳，J.B.，鲁宾，C.S.，以及罗森，O.M.（1979年）《美国国家科学院院刊》76，2725 - 2729）。用胰岛素处理过的细胞提取物在体外也表现出将[γ - ³²P]ATP中的³²P掺入蛋白S6的能力增强（史密斯，C.J.，鲁宾，C.S.，以及罗森，O.M.（1980年）《美国国家科学院院刊》77，2641 - 2645）。我们现在报告，将胰岛素直接添加到含有高亲和力胰岛素受体和来自胰岛素敏感细胞（之前未接触过胰岛素）的核糖体的微粒体制剂中，可刺激[γ - ³²P]ATP中的³²P掺入核糖体蛋白S6，增幅为1.5至3.0倍。这种刺激在与完整细胞相同浓度的胰岛素（0.1 - 1.0 nM）下以及相同时间段内发生。
