Gal'tsova R D, Vakina I P
Mikrobiologiia. 1981 Jan-Feb;50(1):84-9.
The content of sterols in yeast organisms changes noticeably depending on the cultivation conditions. This content sharply decreases when the cells are transferred from aerobic to anaerobic conditions. The proportion between various sterol fractions also changes. The content of bound sterols under aerobic conditions reaches 85-90% of the total sterol content; that of free sterols is only 10-15%. Under anaerobic conditions, the quantity of bond sterols decreases to 70-75% while that of free sterols increases respectively to 25-30%. The fraction of free sterols increases whereas that of bound sterols decreases under aerobic conditions if oleic acid is added to the cultural broth. The content of all of the sterol fractions increases if oleic acid is added under anaerobic conditions. The same correlation is found when ergosterol is added to the medium under anaerobic conditions. Only the total sterol content and the content of sterol esters increase, rather than the content of free sterols, when cholesterol is added to the medium under anaerobic conditions.
酵母生物体中甾醇的含量会根据培养条件而显著变化。当细胞从需氧条件转移到厌氧条件时，该含量会急剧下降。各种甾醇组分之间的比例也会发生变化。在需氧条件下，结合甾醇的含量达到总甾醇含量的85 - 90%；游离甾醇的含量仅为10 - 15%。在厌氧条件下，结合甾醇的量减少到70 - 75%，而游离甾醇的量则分别增加到25 - 30%。如果在培养液中添加油酸，在需氧条件下游离甾醇的组分增加而结合甾醇的组分减少。如果在厌氧条件下添加油酸，所有甾醇组分的含量都会增加。在厌氧条件下向培养基中添加麦角甾醇时也会发现相同的相关性。当在厌氧条件下向培养基中添加胆固醇时，只有总甾醇含量和甾醇酯的含量增加，而游离甾醇的含量不增加。
