Matulaĭtite E Iu, Avizhenis V Iu, Ianulaĭtene A K, Geguzhene A A
Prikl Biokhim Mikrobiol. 1980 Jul-Aug;16(4):528-37.
Intracellular invertase was isolated from the yeast Saccharomyces cerevisiae, race XI, and purified by ion-exchange chromatography on DEAE-cellulose and gel-filtration on Sephadex G-200. The effect of pH, temperature, metal ions, thiolic agents, and EDTA on the enzyme activity and stability was investigated. The enzyme was estimated to have a molecular weight of 270 000 and a carbohydrate content of 20--30%. By disc-electrophoresis and isoelectric focusing the highly purified enzyme was found to be heterogenous. Its molecular forms had isoelectric points at 3.0, 4.0, 4.5, and 4.9.
从酿酒酵母XI族中分离出细胞内转化酶，并通过在DEAE - 纤维素上进行离子交换色谱和在Sephadex G - 200上进行凝胶过滤进行纯化。研究了pH、温度、金属离子、硫醇试剂和EDTA对该酶活性和稳定性的影响。估计该酶的分子量为270000，碳水化合物含量为20 - 30%。通过圆盘电泳和等电聚焦发现，高度纯化的酶是不均一的。其分子形式的等电点分别为3.0、4.0、4.5和4.9。
