Tiller F W, Diener E
Zentralbl Bakteriol A. 1981 Feb;248(4):488-93.
A direct sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect Haemophilus influenzae type b capsular antigen. Using polystyrene as the solid phase and peroxidase-labelled rabbit antibody the assay detected the antigen in concentrations of 0.1 ng/ml. Linearity was achieved within the range of 1ng to 10 microgram/ml. Subtle measurements of Haemophilus influenzae type b capsular antigen in body fluids are possible through ELISA which is superior to counterimmunoelectrophoresis and latex-particle agglutination in this respect. ELISA should facilitate investigations concerning PRP pathogenic effects in experimental Hib infection as well as in human Hib disease.
已开发出一种直接夹心酶联免疫吸附测定法（ELISA）来检测b型流感嗜血杆菌的荚膜抗原。该测定法以聚苯乙烯为固相，使用过氧化物酶标记的兔抗体，可检测浓度为0.1 ng/ml的抗原。在1 ng至10微克/毫升的范围内实现了线性关系。通过ELISA可以对体液中的b型流感嗜血杆菌荚膜抗原进行精细测量，在这方面它优于对流免疫电泳和乳胶颗粒凝集试验。ELISA应有助于对实验性b型流感嗜血杆菌感染以及人类b型流感嗜血杆菌疾病中聚核糖磷酸致病作用的研究。
