Bunge R P, Bunge M B
J Cell Biol. 1978 Sep;78(3):943-50. doi: 10.1083/jcb.78.3.943.
Explants of fetal rat sensory ganglia, cultured under conditions allowing axon and Schwann cell outgrowth in the absence of fibroblasts, occasionally develop nerve fascicles that are partially suspended in culture medium above the collagen substrate. In these suspended regions, fascicles are abnormal in that Schwann cells are decreased in number, are confined to occasional clusters along the fascicle, provide ensheathment for only a few axons at the fascicle periphery, and do not form myelin. When these fascicles are presented with a substrate of reconstituted rat-tail collagen, Schwann cell numbers increase, ensheathment of small nerve fibers occurs normally, and larger axons are myelinated. We conclude that, for normal development, Schwann cells require contact with extracellular matrix as well as axons. The Schwann cell abnormalities in suspended fascicles are similar to those observed in nerve roots of dystrophic mice.
在无成纤维细胞的情况下，于允许轴突和施万细胞生长的条件下培养的胎鼠感觉神经节外植体，偶尔会形成部分悬浮于胶原底物上方培养基中的神经束。在这些悬浮区域，神经束存在异常，表现为施万细胞数量减少，局限于沿神经束偶尔出现的细胞簇中，仅为神经束周边的少数轴突提供髓鞘形成，且不形成髓磷脂。当给这些神经束提供重组大鼠尾胶原底物时，施万细胞数量增加，小神经纤维的髓鞘形成正常，较大的轴突也会形成髓鞘。我们得出结论，对于正常发育，施万细胞既需要与细胞外基质接触，也需要与轴突接触。悬浮神经束中的施万细胞异常与营养不良小鼠神经根中观察到的异常相似。
