Salzer J L, Williams A K, Glaser L, Bunge R P
J Cell Biol. 1980 Mar;84(3):753-66. doi: 10.1083/jcb.84.3.753.
When prepared by methods utilized in our laboratory, pure populations of Schwann cells in culture do not divide, but, after recombination with peripheral sensory neurons or their processes, proliferate rapidly (Wood and Bunge, 1975, Nature (Lond.) 256:661--664). In this paper, we demonstrate that a membrane fraction prepared from sensory ganglion neurites is also mitogenic for Schwann cells and increases the labeling index (assessed by autoradiography after incubation of cells with tritiated thymidine) from less than 0.2 to 10% for primary cells, and from 0.4 to 18--19% for replated cells. The increased responsiveness of replated cells may reflect their greater access to the neurite membranes which is a consequence of the elimination of multiple cell layers after replating and the removal of the basal lamina. This stimulation was specific; addition of membrane preparations from other cell types (3T3, C1300, etc.) was not mitogenic. Ultrastructural analysis demonstrated apparent binding of neurite membranes to Schwann cells as well as significant phagocytosis of the membranes by the cells. The uptake of nonmitogenic membranes suggests that phagocytosis per se is not the stimulus of proliferation.
当采用我们实验室所使用的方法制备时，培养中的雪旺细胞纯群体不会分裂，但在与外周感觉神经元或其突起重组后，会迅速增殖（伍德和邦奇，1975年，《自然》（伦敦）256:661 - 664）。在本文中，我们证明从感觉神经节神经突制备的膜组分对雪旺细胞也具有促有丝分裂作用，并将原代细胞的标记指数（用氚标记胸腺嘧啶核苷孵育细胞后通过放射自显影评估）从小于0.2%提高到10%，将传代细胞的标记指数从0.4%提高到18% - 19%。传代细胞反应性的增加可能反映出它们更易于接触神经突膜，这是传代后消除多层细胞以及去除基膜的结果。这种刺激具有特异性；添加来自其他细胞类型（3T3、C1300等）的膜制剂没有促有丝分裂作用。超微结构分析表明神经突膜与雪旺细胞之间存在明显的结合，并且细胞对膜有显著的吞噬作用。对无促有丝分裂作用膜的摄取表明吞噬作用本身并非增殖的刺激因素。
