Frank H, Nicholson G J, Bayer E
J Chromatogr. 1978 Sep 1;146(2):197-206. doi: 10.1016/s0378-4347(00)81885-x.
Chirasil-Val, a novel chiral polysiloxane-type stationary phase is capable of separating the enantiomers of optically active drugs and metabolites of several compound classes; alpha-amino acides, alpha-amino alcohols, glycols, aromatic and aliphatic alpha-hydroxy carboxylic acids and amines. Due to their high thermal stability, columns coated with Chirasil-Val may be coupled to a mass spectrometer. Potential applications of the new stationary phase include analysis of the optical purity of enantiomeric drugs, determination of the configuration of metabolites, and quantitation of optically active drugs and metabolites using the unnatural enantiometer as internal standard. Direct separation of enantimoers on Chirasil-Val is especially useful if only minute amounts of the optically active compounds are availalbe for analysis.
Chirasil-Val是一种新型手性聚硅氧烷类固定相，能够分离多种化合物类别的旋光性药物及其代谢物；α-氨基酸、α-氨基醇、二醇、芳香族和脂肪族α-羟基羧酸以及胺类。由于其具有高热稳定性，涂覆有Chirasil-Val的色谱柱可与质谱仪联用。这种新型固定相的潜在应用包括对映体药物光学纯度的分析、代谢物构型的测定，以及使用非天然对映体作为内标对旋光性药物和代谢物进行定量。如果只有微量的旋光性化合物可用于分析，在Chirasil-Val上直接分离对映体特别有用。
