[Biphasic peroxidase in the study of Fc receptors of leukemic lymphocytes].

The authors examined Fc receptors of cytoplasmic membrane by means of a two-stage immunoperoxidase technique: at the first stage the immuno-gamma-globulins (IgG), labelled with peroxidase, were connected with the membrane receptors, but at the second stage the connection was stabilised by supplementation of homologous antigen. In healthy persons (20 volunteers) the percentage of lymphocytes, which carried membrane Fc receptors for IgG, were in the ranges of 25% and 42%. In leukemic lymphocytes, verified as B population by immunofluorescent technique, they found that over 95% of these lymphocytes carried membrane receptors for Fc of IgG (examined in 10 persons with chronic lympholeucosis). It could be concluded that the leukemic process does not involve Fc receptor structures in contrast to immunoglobulin receptors, which in these cases are altered considerably, having in mind the amount of the connected labelled IgG and the way od its location on the cytoplasmic membrane.