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紫外线对携带大肠杆菌lexA基因野生型或突变型等位基因的沙门氏菌的致死和诱变作用

[Lethal and mutagenic action of UV light on salmonellae carrying wild or mutant alleles of the Escherichia coli lexA-gene].

作者信息

Andreeva I V, Tiganova I G, Skavronskaia A G

出版信息

Genetika. 1981;17(1):60-5.

PMID:7014362
Abstract

To elucidate the reasons for the absence of UV-mutability in Salmonella typhimurium, the lexA gene of Escherichia coli has been transduced by phage P1 into S. typhimurium. The functioning of lexA+ allele of E. coli in the chromosome of Salmonella failed to cause UV-mutability of the hybrid. The transfer of pKM101 plasmid into the LexA+ hybrid mediates the expressed UV mutability and UV-protective plasmid effect. This plasmid harboured by the LexA hybrid fails to increase UV-resistance and mutability, thus showing the dependence of plasmid mediated effect on LexA+ phenotype.

摘要

为阐明鼠伤寒沙门氏菌缺乏紫外线可突变性的原因,大肠杆菌的lexA基因已被噬菌体P1转导到鼠伤寒沙门氏菌中。大肠杆菌lexA +等位基因在沙门氏菌染色体中的功能未能导致杂种的紫外线可突变性。将pKM101质粒转移到LexA +杂种中可介导表达的紫外线可突变性和紫外线保护质粒效应。LexA杂种携带的这种质粒未能增加紫外线抗性和可突变性,因此表明质粒介导的效应依赖于LexA +表型。

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