[大肠杆菌与鼠伤寒沙门氏菌的属间接合杂交。II. 多聚腺苷酸聚合酶1（polA1）突变从大肠杆菌向鼠伤寒沙门氏菌的转移及其在沙门氏菌基因组中的表型表达]
[Intergeneric conjugational crossing of Escherichia coli with Salmonella typhimurium. II. Transfer of a polA1 mutation from Escherichia coli to Salmonella typhimurium and its phenotypic expression in the salmonella genome].
作者信息
Stepanova N F, Andreeva I V, Kiriushkina A A, Kondrat'ev Iu S, Likhoded L Ia
出版信息
Genetika. 1977;13(12):2189-94.
The Escherichia coli structural gene for DNA polymerase I was inserted into Salmonella typhimurium chromosome by conjugal transfer. The genetic analysis of P1-mediated transduction of obtained hybrid showed that polA gene is located in it between metE and rha loci and is cotransduced with metE (about 50%) and rha (12%). The phenotypic properties of polA1 hybrid E. coliXS. typhimurium concerning UV-MMS-NG and gamma-ray sensitivity are similar to the polA1 mutants of E. coli.
通过接合转移将大肠杆菌DNA聚合酶I的结构基因插入鼠伤寒沙门氏菌染色体。对获得的杂种进行P1介导转导的遗传分析表明，polA基因位于metE和rha基因座之间，与metE（约50%）和rha（12%）共转导。关于紫外线-甲基磺酸甲酯-氮芥和γ射线敏感性，polA1杂种大肠杆菌X鼠伤寒沙门氏菌的表型特性与大肠杆菌的polA1突变体相似。
Zotero 插件