Harris G, Browne C, Cramp W A
Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Jan;39(1):9-19. doi: 10.1080/09553008114550021.
Explants of rabbit spleen, responding to sheep erythrocytes in vitro, were exposed to ionizing radiation at different times in culture. Radiation was found to depress the development of specific haemolysin-producing cells (PFC) at subsequent times, while cells already engaged in haemolysin production were relatively resistant to radiation. The depressing effects of radiation on PFC was most marked at times when the rate of increase of PFC was highest. Recovery from low doses (less than 2 Gy) occurred while higher doses of radiation had persistent depressing effects on the level of PFC. Assessment of synthesis of other macromolecules in these irradiated cultures showed that protein synthesis was highly resistant and DNA synthesis was sensitive like the immune response. Irradiation in hypoxic conditions showed that this protected the tissues from damage by radiation and there were also indications of enhancement of repair of this damage. Finally it was found that the results obtained with these cultures correlated well with previous studies of intact mice.
兔脾外植体在体外对绵羊红细胞产生反应，在培养的不同时间接受电离辐射。结果发现，辐射会在随后的时间抑制特定溶血素产生细胞（PFC）的发育，而已经参与溶血素产生的细胞对辐射相对具有抗性。辐射对PFC的抑制作用在PFC增加速率最高时最为明显。低剂量（小于2 Gy）辐射后的恢复会发生，而更高剂量的辐射对PFC水平具有持续的抑制作用。对这些受辐照培养物中其他大分子合成的评估表明，蛋白质合成具有高度抗性，DNA合成与免疫反应一样敏感。在缺氧条件下进行辐照表明，这可保护组织免受辐射损伤，并且也有迹象表明这种损伤的修复得到增强。最后发现，这些培养物获得的结果与先前对完整小鼠的研究结果高度相关。
