细胞松弛素将微管解聚与不对称形态的丧失区分开来。
Cytochalasin separates microtubule disassembly from loss of asymmetric morphology.
作者信息
Solomon F, Magendantz M
出版信息
J Cell Biol. 1981 Apr;89(1):157-61. doi: 10.1083/jcb.89.1.157.
Abstract
When neuroblastoma cells bearing neurites are incubated with colchicine or Nocodazole, the cytoplasmic microtubules are depolymerized and concomitantly the neurites retract. We report here that cytochalasin separates the two effects of these drugs: it quantitatively inhibits neurite retraction but does not inhibit microtubule assembly. The neurites that remain contain intermediate filaments and actin but are devoid of microtubules. Depletion of cellular ATP also blocks neurite retraction induced by colchicine or Nocodazole, but some assembled microtubules persist under these conditions. The results suggest that neurite retraction is an active cell process.
摘要
当带有神经突的神经母细胞瘤细胞与秋水仙碱或诺考达唑一起孵育时，细胞质微管会解聚，神经突也会随之回缩。我们在此报告，细胞松弛素可区分这些药物的两种作用：它能定量抑制神经突回缩，但不抑制微管组装。残留的神经突含有中间丝和肌动蛋白，但没有微管。细胞ATP的耗尽也会阻断秋水仙碱或诺考达唑诱导的神经突回缩，但在这些条件下仍有一些组装好的微管存在。结果表明，神经突回缩是一个活跃的细胞过程。
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