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通过与辛二酸二甲酯亚胺和二甲基-3,3'-二硫代双丙酰亚胺交联确定的大肠弯曲杆菌5S RNA-蛋白质复合物的拓扑结构。

Topography of the C. coli 5S RNA-protein complex as determined by crosslinking with dimethyl suberimidate and dimethyl-3,3'-dithiobispropionimidate.

作者信息

Fanning T G, Traut R R

出版信息

Nucleic Acids Res. 1981 Feb 25;9(4):993-1004. doi: 10.1093/nar/9.4.993.

DOI:10.1093/nar/9.4.993
PMID:7015289
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326728/
Abstract

5S RNA-protein complexes were prepared in vitro using partially purified E. coli 5S RNA and total E. coli 70S ribosomal proteins. The complexes were isolated from sucrose gradients and shown to contain proteins L5, L18, L25 and a fourth protein not heretofore characterized and designed L31. The complexes were treated with the crosslinking reagents dimethyl suberimidate and dimethyl-3,3'-dithiobispropionimidate. Both reagents gave identical patterns of crosslinked proteins when analyzed by one-dimensional polyacrylamide/dodecylsulfate gel electrophoresis. Dimers of L5-L31', L5-L18 and L18-L18 and a trimer containing L5, L18 and L31' were identified by diagonal polyacrylamide/dodecylsulfate gel electrophoresis of the proteins crosslinked with dimethyl-3,3'-dithiobispropionimidate. No crosslinking was detected between L25 and the other three proteins.

摘要

使用部分纯化的大肠杆菌5S RNA和总的大肠杆菌70S核糖体蛋白在体外制备5S RNA - 蛋白质复合物。这些复合物从蔗糖梯度中分离出来，并显示含有蛋白质L5、L18、L25以及第四种迄今未鉴定的蛋白质，命名为L31。用交联试剂亚胺基二琥珀酸二甲酯和二甲基 - 3,3'-二硫代双丙酰亚胺处理这些复合物。当通过一维聚丙烯酰胺/十二烷基硫酸盐凝胶电泳分析时，两种试剂产生相同的交联蛋白模式。通过对用二甲基 - 3,3'-二硫代双丙酰亚胺交联的蛋白质进行对角线聚丙烯酰胺/十二烷基硫酸盐凝胶电泳，鉴定出L5 - L31'、L5 - L18和L18 - L18的二聚体以及包含L5、L18和L31'的三聚体。未检测到L25与其他三种蛋白质之间的交联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/e5fcc7403861/nar00397-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/2428e45220c9/nar00397-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/0092fbf24e0e/nar00397-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/f8502f828679/nar00397-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/118b90e0928f/nar00397-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/e5fcc7403861/nar00397-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/2428e45220c9/nar00397-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/0092fbf24e0e/nar00397-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/f8502f828679/nar00397-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/118b90e0928f/nar00397-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5c/326728/e5fcc7403861/nar00397-0251-a.jpg

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.大肠杆菌中指定核糖体蛋白的染色体位点。
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1367-74. doi: 10.1073/pnas.52.6.1367.
3
Isolation, purification and properties of 5 s ribosomal RNA: a new species of cellular RNA.
J Mol Biol. 1967 Feb 14;23(3):441-58. doi: 10.1016/s0022-2836(67)80117-7.
Adv Sci (Weinh). 2018 Jul 30;5(10):1800614. doi: 10.1002/advs.201800614. eCollection 2018 Oct.
4
The evolutionary history of the structure of 5S ribosomal RNA.5S 核糖体 RNA 结构的进化历史。
J Mol Evol. 2009 Nov;69(5):430-43. doi: 10.1007/s00239-009-9264-z. Epub 2009 Jul 29.
5
MrpL36p, a highly diverged L31 ribosomal protein homolog with additional functional domains in Saccharomyces cerevisiae mitochondria.MrpL36p，一种在酿酒酵母线粒体中高度分化的L31核糖体蛋白同源物，带有额外的功能结构域。
Genetics. 2004 May;167(1):65-75. doi: 10.1534/genetics.167.1.65.
6
Secondary structure model for 23S ribosomal RNA.23S核糖体RNA的二级结构模型
Nucleic Acids Res. 1981 Nov 25;9(22):6167-89. doi: 10.1093/nar/9.22.6167.
7
Structural analyses of E. coli 5S RNA fragments, their associates and complexes with proteins L18 and L25.大肠杆菌5S RNA片段、其与蛋白质L18和L25的缔合物及复合物的结构分析。
Nucleic Acids Res. 1982 Feb 11;10(3):947-65. doi: 10.1093/nar/10.3.947.
4
Accessibility of 5S RNA to ribonucleases in Escherichia coli ribosomes.
Biochimie. 1971;53(5):657-60. doi: 10.1016/s0300-9084(71)80021-4.
5
Ribosomal proteins of Escherichia coli. II. Proteins from the 30 s subunit.大肠杆菌的核糖体蛋白。II. 来自30S亚基的蛋白。
J Mol Biol. 1968 Feb 14;31(3):441-61. doi: 10.1016/0022-2836(68)90420-8.
6
Diagonal polyacrylamide-dodecyl sulfate gel electrophoresis for the identification of ribosomal proteins crosslinked with methyl-4-mercaptobutyrimidate.
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3946-50. doi: 10.1073/pnas.71.10.3946.
7
Identification by photo-affinity labeling of the proteins in Escherichia coli ribosomes involved in elongation factor G-dependent GDP binding.通过光亲和标记鉴定大肠杆菌核糖体中参与延伸因子G依赖性GDP结合的蛋白质。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1277-80. doi: 10.1073/pnas.71.4.1277.
8
Identification of the nucleotide sequences involved in the interaction between Escherichia coli 5 RNA and specific 50 S subunit proteins.鉴定大肠杆菌5 RNA与特定50 S亚基蛋白之间相互作用所涉及的核苷酸序列。
J Mol Biol. 1973 Jun 15;77(1):133-52. doi: 10.1016/0022-2836(73)90367-7.
9
The sequence of steps in the attachment of 5-S RNA to cores of Escherichia coli ribosomes.5-S RNA与大肠杆菌核糖体核心结合的步骤顺序。
Biochim Biophys Acta. 1973 Oct 26;324(3):375-85. doi: 10.1016/0005-2787(73)90282-7.
10
Specific recognition of GTpsiC loop (loop IV) of tRNA by 50S ribosomal subunits from E. coli.来自大肠杆菌的50S核糖体亚基对tRNA的GTpsiC环（环IV）的特异性识别。
Nat New Biol. 1973 Dec 5;246(153):132-5. doi: 10.1038/newbio246132a0.