Kishigami Y, Noda S, Morishita T, Hashimoto Y
Anal Quant Cytol. 1981 Mar;3(1):43-8.
An improved method of preparing vaginal smears for use in the Auto-Cyto-Screener is reported. The main alterations from the previous technique were the substitution of 2% formaldehyde phosphate buffer (pH 7.4) for 50% ethanol as the preservative fluid, the use of homogenization in place of sonication for cell aggregate dispersion and the introduction of Ficoll-gradient sedimentation, which not only removes leukocytes and other elements from the suspensions but also concentrates the cancer cells to some extent. An examination of cell deposition techniques showed that optimal results were achieved with the use of filter paper, not rubber, and with centrifugation performed immediately after loading the suspension into the chambers.
本文报道了一种改进的制备用于自动细胞筛选仪的阴道涂片的方法。与先前技术的主要改动包括：用2%甲醛磷酸盐缓冲液（pH 7.4）替代50%乙醇作为保存液；使用匀浆法代替超声处理来分散细胞聚集体；引入Ficoll梯度沉降法，该方法不仅能从悬浮液中去除白细胞和其他成分，还能在一定程度上浓缩癌细胞。对细胞沉积技术的研究表明，使用滤纸而非橡胶，并在将悬浮液加载到腔室后立即进行离心，可获得最佳结果。
