Filice G, Carnevale G, Meroni V, Lanzarini P, Carosi G
Boll Ist Sieroter Milan. 1980;59(6):604-11.
Mice were infected with a "cystogenic" strain of T. gondii and sera obtained at different times were tested using DT and IFAT (cell wall antibodies tests) and IHAT (performed with extractive cytoplasmic antigen). We found different kinetics of DT- and IFAT- versus IHAT-titers: IHAT is negative during the early phase of the experimental infection when DT and IgM-IFAT are already positive. Furthermore IgM antibodies were never found against cytoplasmic antigens by the IHAT method. At weekly intervals after the experimental infection a biological test was also performed using samples of different organs. We found that one week after the infection only the brain was constantly positive in the biological test. The antibody pattern was similar in every positive test irrespective of the source and size of the material used for the inoculum. These data suggest that the result of a biological test may be available earlier (already after 7 days) if a test for antibodies against cell wall antigens is performed on the IgM fraction of the mouse serum.
将小鼠感染“产囊型”刚地弓形虫菌株，并使用直接凝集试验（DT）和间接荧光抗体试验（IFAT，细胞壁抗体检测）以及间接血凝试验（IHAT，使用提取的细胞质抗原进行）对不同时间获取的血清进行检测。我们发现DT和IFAT与IHAT效价的动力学不同：在实验性感染的早期阶段，当DT和IgM-IFAT已经呈阳性时，IHAT呈阴性。此外，通过IHAT方法从未发现针对细胞质抗原的IgM抗体。在实验性感染后每隔一周，还使用不同器官的样本进行生物学检测。我们发现感染后一周，仅大脑在生物学检测中持续呈阳性。无论用于接种物的材料来源和大小如何，每次阳性检测中的抗体模式都是相似的。这些数据表明，如果对小鼠血清的IgM部分进行针对细胞壁抗原的抗体检测，生物学检测结果可能会更早获得（7天后即可）。
