抗神经元细胞表面糖蛋白的单克隆抗体。

Monoclonal antibody against cell surface glycoprotein of neurons.

作者信息

Hirn M, Pierres M, Deagostini-Bazin H, Hirsch M, Goridis C

出版信息

Brain Res. 1981 Jun 15;214(2):433-9. doi: 10.1016/0006-8993(81)91208-7.

Abstract

A monoclonal antibody, named BSP-2, has been produced against glycoproteins extracted from neonatal mouse brain. Its reactivity with live cells established the surface location of the antigen. In primary cultures of dissociated cerebellar cells, the antibody bound to neuronal cell types, but not to astrocytes nor to fibroblasts. Immunoprecipitates prepared with the BSP-2 antibody contained a triplet of high-molecular weight glycoproteins with apparent molecular weights of 180,000, 140,000 and 120,000.

摘要

一种名为BSP - 2的单克隆抗体已针对从小鼠新生大脑中提取的糖蛋白制备而成。其与活细胞的反应性确定了抗原的表面位置。在解离的小脑细胞原代培养物中，该抗体与神经元细胞类型结合，但不与星形胶质细胞或成纤维细胞结合。用BSP - 2抗体制备的免疫沉淀物包含一组高分子量糖蛋白，其表观分子量分别为180,000、140,000和120,000。