Langley O K, Ghandour M S, Gombos G, Hirn M, Goridis C
Neurochem Res. 1982 Mar;7(3):349-62. doi: 10.1007/BF00965646.
A comparison is made of the immunohistochemistry at the ultrastructural level of three monoclonal antibodies directed against surface components of DNS cells. Hybridomas secreting these antibodies were obtained from two cell fusions of a rat myeloma cell line and immune splenocytes derived from rats immunized either with primary mouse brain cultured cells or membrane components. In cultures one antibody, anti-BSP2 (Brain Surface Protein-2), was preferentially directed against neurons while another, anti-BSP-3 (Brain Surface Protein-3), preferentially labeled astrocytes. In mouse cerebellar sections, both labeled the surface of Purkinje cells, granule cells and astrocytes. In addition a cytoplasm localization was apparent in granule cells and astrocytes. Another antibody anti-MESA-1 (Mouse Endothelial Surface Antigen-1) reacted exclusively with the surface of endothelial cells lining blood vessels. These data are discussed with reference to the biochemical nature of the corresponding antigens and to known glycoproteins of neural cell membranes.
对三种针对DNS细胞表面成分的单克隆抗体在超微结构水平上的免疫组织化学进行了比较。分泌这些抗体的杂交瘤是从大鼠骨髓瘤细胞系与分别用原代小鼠脑培养细胞或膜成分免疫的大鼠的免疫脾细胞进行的两次细胞融合中获得的。在培养物中，一种抗体抗BSP2（脑表面蛋白-2）优先针对神经元，而另一种抗体抗BSP-3（脑表面蛋白-3）则优先标记星形胶质细胞。在小鼠小脑切片中，两者都标记了浦肯野细胞、颗粒细胞和星形胶质细胞的表面。此外，在颗粒细胞和星形胶质细胞中可见细胞质定位。另一种抗体抗MESA-1（小鼠内皮表面抗原-1）仅与血管内皮细胞表面发生反应。结合相应抗原的生化性质以及神经细胞膜的已知糖蛋白对这些数据进行了讨论。
