Ishiguro E E, Ramey W D
Can J Microbiol. 1980 Dec;26(12):1514-8. doi: 10.1139/m80-253.
Physiological concentrations of guanosine 5'-diphosphate 3'-diphosphate (ppGpp) inhibited the synthesis of lipid intermediates and peptidoglycan catalyzed by a particulate enzyme preparation from Escherichia coli. The inhibition of these reactions was dependent on the concentrations of ppGpp and MgCl2 in the assay. The degree of inhibition of lipid intermediate synthesis decreased as the molar ratio of MgCl2 to ppGpp was increased, and no inhibition was observed above a MgCl2 to ppGpp ratio of 2.5. The synthesis of peptidoglycan was more sensitive to inhibition by ppGpp, and significant inhibition occurred under conditions where lipid intermediate synthesis was unaffected (i.e., at MgCl2 to ppGpp ratios of 2.5 or more). A variety of other nucleotides did not inhibit the synthesis of lipid intermediates and peptidoglycan.
5'-二磷酸鸟苷-3'-二磷酸（ppGpp）的生理浓度抑制了由大肠杆菌颗粒酶制剂催化的脂质中间体和肽聚糖的合成。这些反应的抑制作用取决于测定中ppGpp和MgCl2的浓度。随着MgCl2与ppGpp的摩尔比增加，脂质中间体合成的抑制程度降低，当MgCl2与ppGpp的比例高于2.5时未观察到抑制作用。肽聚糖的合成对ppGpp的抑制更敏感，并且在脂质中间体合成未受影响的条件下（即MgCl2与ppGpp的比例为2.5或更高时）会发生显著抑制。多种其他核苷酸不抑制脂质中间体和肽聚糖的合成。
