Lechner J F, Haugen A, Autrup H, McClendon I A, Trump B F, Harris C C
Cancer Res. 1981 Jun;41(6):2294-304.
Normal primary epithelial cell cultures devoid of fibroblastic cells have been developed from tissue explants of adult human bronchi. Conditions for clonal growth of secondary cultures of bronchial epithelial cells were optimized by coculturing the human cells with mitomycin C growth-arrested Swiss 3T3 mouse feeder cells, lowering the calcium concentration of medium M199, and supplementing it with hydrocortisone, insulin, cholera toxin, epidermal growth factor, and 1.25% fetal bovine serum. The epithelial cells grew for an average of 35 population doublings and had the normal human karyotype, expressed keratin and blood group antigen epithelial cell markers, metabolized benzo(a)pyrene, and were capable of differentiating into both ciliated and squamous cells. This culture system makes it potentially possible to investigate various aspects of differentiation and carcinogenesis in human bronchial epithelial cells.
已从成人人类支气管组织外植体中培养出无成纤维细胞的正常原代上皮细胞。通过将人类细胞与丝裂霉素C处理使其生长停滞的瑞士3T3小鼠饲养细胞共培养、降低M199培养基的钙浓度，并添加氢化可的松、胰岛素、霍乱毒素、表皮生长因子和1.25%胎牛血清，优化了支气管上皮细胞传代培养的克隆生长条件。上皮细胞平均生长35代，具有正常的人类核型，表达角蛋白和血型抗原上皮细胞标志物，代谢苯并(a)芘，并且能够分化为纤毛细胞和鳞状细胞。该培养系统使得研究人类支气管上皮细胞分化和致癌作用的各个方面成为可能。
