Shannon B T, Love S H
Immunol Commun. 1980;9(8):735-46. doi: 10.3109/08820138009109683.
A slight macrophage disappearance reaction was elicited in C3H/Hej mice, a low responder strain. Low levels of hyaluronic acid accumulated in the peritoneal fluid during the disappearance reaction, in keeping with the low response. The macrophage disappearance reaction was not simulated by the intraperitoneal injection of large amounts of hyaluronate into nonsensitized C3H/Hej mice. This suggested that these macrophages were refractory to hyaluronic acid. Subsequent experiments demonstrated that low concentrations of hyaluronic acid did not agglutinate these cells, in vitro, nor inhibit their migration to the extent obtained with macrophages from the responder mice (C57BL/6 strain), thus providing evidence that the low responder mice had at least two defects with respect to the disappearance reaction: 1) they accumulated less hyaluronic acid in their peritoneal fluid, and 2) their macrophages were refractory towards hyaluronic acid. Generalized defects in membranes of various cell types of C3H/Hej mice, involving receptors for lymphokines in the case of mesothelial cells, or hyaluronic acid receptors on macrophages may serve as the central defect and explain the above observations.
在低反应性品系C3H/Hej小鼠中引发了轻微的巨噬细胞消失反应。在消失反应期间，腹膜液中积累了低水平的透明质酸，这与低反应性一致。通过向未致敏的C3H/Hej小鼠腹腔注射大量透明质酸盐，并未模拟出巨噬细胞消失反应。这表明这些巨噬细胞对透明质酸具有抗性。随后的实验表明，低浓度的透明质酸在体外既不会凝集这些细胞，也不会像在反应性小鼠（C57BL/6品系）的巨噬细胞中那样抑制其迁移，从而提供了证据，证明低反应性小鼠在消失反应方面至少存在两个缺陷：1）它们在腹膜液中积累的透明质酸较少，2）它们的巨噬细胞对透明质酸具有抗性。C3H/Hej小鼠各种细胞类型的膜存在普遍缺陷，对于间皮细胞而言涉及淋巴因子受体，对于巨噬细胞而言涉及透明质酸受体，这可能是核心缺陷，并解释了上述观察结果。
