Buchanan D, Kamarck M, Ruddle N H
J Immunol Methods. 1981;42(2):179-85. doi: 10.1016/0022-1759(81)90147-2.
A protein A enzyme immunoassay has been developed which is effective in the rapid screening of hybridomas. The detecting reagent is a stable alkaline phosphatase-protein A conjugate, prepared by a simple, inexpensive, single-step procedure. The assay requires small amounts of antigen to coat microtiter wells (less than 25 microgram/well) and can be evaluated visually or with a spectrophotometer. It compares favorably in sensitivity to a protein A-radioimmunoassay and enjoys considerably lower backgrounds than that popular screening procedure.
已开发出一种蛋白A酶免疫测定法，可有效快速筛选杂交瘤。检测试剂是一种稳定的碱性磷酸酶-蛋白A偶联物，通过简单、廉价的单步程序制备。该测定法只需少量抗原包被微量滴定板孔（每孔少于25微克），可通过目视或用分光光度计进行评估。其灵敏度与蛋白A放射免疫测定法相当，且背景比那种常用的筛选方法低得多。
