一种用于鉴定未浓缩脑脊液中寡克隆免疫球蛋白的灵敏方法。
A sensitive method for characterization of oligoclonal immunoglobulins in unconcentrated cerebrospinal fluid.
作者信息
Lasne Y, Benzerara O, Chazot G, Creyssel R
出版信息
J Neurochem. 1981 May;36(5):1872-4. doi: 10.1111/j.1471-4159.1981.tb00445.x.
Abstract
Although isoelectric focusing has been used to demonstrate the presence of oligoclonal IgG in the CSF, the technique has not allowed detection of oligoclonal IgG in unconcentrated CSF. A new technique is reported, by which unconcentrated CSF is separated by isoelectric focusing, and the IgG bands are then detected by radioimmunofixation. Samples as small as 20 microliters may be used.
摘要
尽管等电聚焦已被用于证明脑脊液中寡克隆IgG的存在，但该技术无法检测未浓缩脑脊液中的寡克隆IgG。本文报道了一种新技术，通过该技术，未浓缩的脑脊液经等电聚焦分离，然后通过放射免疫固定检测IgG条带。样本量小至20微升即可使用。
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