Beyer E C, Barondes S H
J Supramol Struct. 1980;13(2):219-27. doi: 10.1002/jss.400130210.
A lactose-binding lectin previously purified from embryonic chicken muscle and adult chicken liver, and here referred to as chicken-lactose-lectin-I (CLL-I), was added to sections of various adult chicken tissues to detect available binding sites. Both the sites of binding of added CLL-I as well as the tissue distribution of endogenous CLL-I were determined by indirect immunofluorescence using a rabbit antibody to CLL-I followed by fluorescent goat anti-rabbit IgG. Some tissues such as intestine and kidney showed abundant extracellular binding sites for the lectin, primarily between cells, in basement membrane, and in material on the luminal surface. In contrast, adult heart showed no significant binding sites for CLL-I. Adult pancreas showed considerable endogenous CLL-I in an extracellular site surrounding exocrine lobules, but added CLL-I did not bind substantially. The distribution of CLL-I binding sites in intestine were mimicked by those of purpurin, another lactose-binding lectin. CLL-I binding sites were also detected on the surface of cultured chick embryo skin fibroblasts. The factors controlling the specific distribution of occupied and unoccupied CLL-I binding sites are not known.
一种先前从胚胎鸡肌肉和成年鸡肝脏中纯化出来的乳糖结合凝集素，在此称为鸡乳糖凝集素-I（CLL-I），被添加到各种成年鸡组织切片中以检测可用的结合位点。通过使用抗CLL-I的兔抗体，随后用荧光山羊抗兔IgG进行间接免疫荧光，确定添加的CLL-I的结合位点以及内源性CLL-I的组织分布。一些组织，如肠道和肾脏，显示出该凝集素丰富的细胞外结合位点，主要在细胞之间、基底膜以及腔表面的物质中。相比之下，成年心脏未显示出CLL-I显著的结合位点。成年胰腺在外分泌小叶周围的细胞外位点显示出相当数量的内源性CLL-I，但添加的CLL-I并未大量结合。肠道中CLL-I结合位点的分布与另一种乳糖结合凝集素紫红素的分布相似。在培养的鸡胚皮肤成纤维细胞表面也检测到了CLL-I结合位点。控制占据和未占据的CLL-I结合位点特定分布的因素尚不清楚。
