Chromosome replication following a temporary inhibition of DNA-synthesis by nalidixic acid in a temperature-sensitive dnaA mutant of Escherichia coli.

Chromosome replication cycle in a DNA initiation mutant of Escherichia coli (CRT-83, dnaAts) was blocked by nalidixic acid, an inhibitor of the A subunit of DNA gyrase. Following a period of inhibition of DNA synthesis, the drug was removed and "run-out" DNA synthesis was examined. It was found that the "capacity" for DNA synthesis was not affected by such a treatment.