异烟肼在沙门氏菌中的致突变性及其对人成纤维细胞DNA修复和合成的影响。

The mutagenicity of isoniazid in Salmonella and its effects on DNA repair and synthesis in human fibroblasts.

作者信息

Wade D R, Lohman P H, Mattern I E, Berends F

出版信息

Mutat Res. 1981 May;89(1):9-20. doi: 10.1016/0165-1218(81)90126-9.

Abstract

A commercial sample of the tuberculostatic drug isoniazid (INH) was found to have a weak mutagenic activity towards Salmonella typhimurium strains TA100 and TA1535. The addition of a rat or mouse liver homogenate to the test system decreased the mutagenic effect of INH. Hydrazine, an impurity of the INH sample, was also weakly mutagenic in strains TA100 and TA1535, but not in the extent that could account for the mutagenicity of the INH sample. An inhibition of DNA synthesis in human fibroblasts was observed for INH, this effect being potentiated by the addition of manganese to the test system. There was no induction of unscheduled DNA synthesis in human cells by INH except in the presence of manganese.

摘要

一种抗结核药物异烟肼（INH）的商业样品被发现对鼠伤寒沙门氏菌TA100和TA1535菌株具有微弱的诱变活性。向测试系统中添加大鼠或小鼠肝脏匀浆可降低INH的诱变作用。肼是INH样品中的一种杂质，在TA100和TA1535菌株中也具有微弱的诱变作用，但程度不足以解释INH样品的诱变性。观察到INH对人成纤维细胞的DNA合成有抑制作用，在测试系统中添加锰可增强这种作用。除了在有锰存在的情况下，INH在人细胞中不会诱导非预定DNA合成。