Kashiwazaki M, Akaike Y, Miyachi T, Ogawa T, Sugawara M, Isayama Y
Natl Inst Anim Health Q (Tokyo). 1981 Spring;21(1):21-5.
Forty-nine strains of Escherichia coli isolated from piglets with or without neonatal diarrhea were all negative for both classical heat-labile and heat-stable enterotoxins when examined by the 18-hour rabbit ileal loop test with culture supernatants, the mouse Y1 adrenal cell test, and the infant mouse test. Of them, seven strains from diarrhea were positive only when tested in porcine ileal loops challenged with living bacterial cell suspensions or culture supernatants. The enterotoxic component produced by them was resistant to heating at 80 degrees C for 20 minutes. It presented a rapid onset of action with a long duration, although the amount of accumulated fluid in the porcine ileal loops was small. These porcine strains of E. coli which has been positive only for the porcine ileal loop test were designated "ST pig loop" or "STpl" strains.
从有或没有新生腹泻的仔猪中分离出的49株大肠杆菌，当用培养上清液进行18小时兔回肠袢试验、小鼠Y1肾上腺细胞试验和幼鼠试验检测时，经典热不稳定和热稳定肠毒素均为阴性。其中，7株来自腹泻仔猪的菌株仅在用活细菌细胞悬液或培养上清液攻击的猪回肠袢试验中呈阳性。它们产生的肠毒素成分在80℃加热20分钟后仍具有活性。尽管猪回肠袢中积聚的液体量较少，但它起效迅速且持续时间长。这些仅在猪回肠袢试验中呈阳性的猪源大肠杆菌菌株被命名为“ST猪回肠袢”或“STpl”菌株。
