Kashiwazaki M, Ogawa T, Nakamura K, Isayama Y, Tamura K, Sakazaki R
Natl Inst Anim Health Q (Tokyo). 1981 Summer;21(2):68-72.
A heat-labile Vero cytotoxin (VT) was detected by the Vero cell assay in the culture supernatants of ten Escherichia coli strains isolated from a variety of clinical sources. The VT titer varied with strains, ranging from 1:8 to 1:2,048. No VT was found to be cytotoxic for Y1 mouse adrenal cells or to induce fluid accumulation in the rabbit or porcine ileal loops. No strains produced a heat-labile enterotoxin as defined by either tissue culture or the standard ileal loop reaction. Three strains originated from piglets with diarrhea were confirmed by the infant mouse reaction to produce a heat-stable enterotoxin. Neutralization studies revealed antigenically the presence of two different forms of VT produced by E. coli strains of animal origin.
通过Vero细胞试验在从各种临床来源分离出的10株大肠杆菌的培养上清液中检测到一种热不稳定的Vero细胞毒素（VT）。VT效价因菌株而异，范围从1:8到1:2,048。未发现VT对Y1小鼠肾上腺细胞具有细胞毒性，也未在兔或猪回肠袢中诱导液体蓄积。按照组织培养或标准回肠袢反应的定义，没有菌株产生热不稳定肠毒素。通过幼鼠反应证实，3株源自腹泻仔猪的菌株产生了一种热稳定肠毒素。中和研究从抗原性上揭示了动物源大肠杆菌菌株产生的两种不同形式VT的存在。
