Cohen B I, Raicht R F
Alcohol Clin Exp Res. 1981 Spring;5(2):225-9. doi: 10.1111/j.1530-0277.1981.tb04893.x.
Sterol metabolism studies using a combination of isotopic and chromatographic procedures were carried out in two strains of rats fed 5% ethanol (36% of calories) in the diet. Feeding ethanol to the Fisher rat over 17 days produced no significant changes in body weight. Cholesterol levels in various tissues were elevated in the ethanol-fed group: plasma cholesterol, +61%; liver cholesterol, +47%; and bile cholesterol, +57%. The alcohol-fed Fisher rat showed several changes in sterol metabolism over controls: fecal acidic steroid output, +13%; fecal neutral sterol output, +51%; endogenous neutral sterol output, +107%; cholesterol turnover, +54%; and cholesterol balance, +18%. Ethanol feeding to the Sprague Dawley rat showed similar differences between ethanol-fed vs. control rats. Cholesterol levels were significantly elevated in plasma (+35%) and in the liver (+81%). Sterol metabolism data showed the following differences (alcohol vs. control): fecal acidic steroid output, +9%; fecal neutral sterol output, +17%; endogenous neutral sterol output, +72%; cholesterol turnover, +33%; and cholesterol balance +13%. The Fisher rat maintained almost constant weight throughout the experimental period and is a preferable strain for sterol balance studies using liquid diets. A major finding of these experiments was the increased concentration of cholesterol in liver, plasma, and bile in both strains of rats. The sterol balance measurements indicated that this tissue accumulation of cholesterol was due to enhanced cholesterol synthesis as well as inhibition of bile acid syntheses.
利用同位素和色谱分析方法相结合，对两组食用含5%乙醇（占卡路里的36%）日粮的大鼠进行了固醇代谢研究。给费希尔大鼠喂食乙醇17天，其体重没有显著变化。乙醇喂养组大鼠各组织中的胆固醇水平均有所升高：血浆胆固醇升高61%；肝脏胆固醇升高47%；胆汁胆固醇升高57%。与对照组相比，喂食乙醇的费希尔大鼠在固醇代谢方面出现了一些变化：粪便酸性类固醇排出量增加13%；粪便中性固醇排出量增加51%；内源性中性固醇排出量增加107%；胆固醇周转率增加54%；胆固醇平衡增加18%。给斯普拉格-道利大鼠喂食乙醇，结果显示乙醇喂养组与对照组大鼠之间也存在类似差异。血浆（升高35%）和肝脏（升高81%）中的胆固醇水平显著升高。固醇代谢数据显示出以下差异（乙醇组与对照组相比）：粪便酸性类固醇排出量增加9%；粪便中性固醇排出量增加17%；内源性中性固醇排出量增加72%；胆固醇周转率增加33%；胆固醇平衡增加13%。费希尔大鼠在整个实验期间体重几乎保持不变，是使用流食进行固醇平衡研究的理想品系。这些实验的一个主要发现是，两种品系大鼠的肝脏、血浆和胆汁中胆固醇浓度均有所增加。固醇平衡测量结果表明，这种组织中胆固醇的积累是由于胆固醇合成增强以及胆汁酸合成受到抑制所致。
