Binoux M, Hossenlopp P, Gourmelen M, Girard F
Ann Endocrinol (Paris). 1980 Nov-Dec;41(6):487-94.
Rat liver explants in culture release into the culture medium IGF and their carrier which form a complex with an apparent molecular weight of similar to or approximately 40,000. With gel filtration in acetic acid the IGF are separated from their carrier and each can then be measured by radioligand assay. In terms of their hormonal control "in vitro", GH, insulin and cortisol have an independent action on each. Pituitary and various brain tissues in organ culture also produce IGF and their carrier. The "in vitro" regulation of the IGF and their carrier has been investigated through assays of their levels in human serum under normal and pathological conditions, such as hypopituitarism, acromegaly, coeliac disease, Cushing's syndrome and idiopathic short or tall stature.
培养中的大鼠肝脏外植体将胰岛素样生长因子（IGF）及其载体释放到培养基中，它们形成一种表观分子量类似于或约为40,000的复合物。通过在乙酸中进行凝胶过滤，可将IGF与其载体分离，然后各自可用放射配体测定法进行测量。就其“体外”激素控制而言，生长激素（GH）、胰岛素和皮质醇对它们各自具有独立作用。器官培养中的垂体和各种脑组织也产生IGF及其载体。通过检测正常和病理条件下（如垂体功能减退、肢端肥大症、乳糜泻、库欣综合征以及特发性身材矮小或高大）人血清中它们的水平，对IGF及其载体的“体外”调节进行了研究。
