Reeve J G, Embleton M J, Baldwin R W
Br J Cancer. 1981 Jun;43(6):809-16. doi: 10.1038/bjc.1981.119.
It has previously been shown that rat embryo cells treated in vitro with 3-methylcholanthrene (MCA) elicit antibodies in syngeneic rats which react specifically against established MCA-induced sarcomas. To examine the possibility that clonal amplification of one or a few antigenic, preneoplastic clones is responsible for the previously observed specific antibody responses, MCA-treated rat embryo cells have been subjected to 150 Gy of gamma-irradiation before injection into host animals. The resulting antisera were screened for reactivity against a panel of established syngeneic tumours by membrane immunofluorescence and an isotopic antiglobulin test. A positive reaction was observed between an antiserum pool raised against gamma-irradiated MCA-treated cells and the cells of an immunogenic spontaneous mammary carcinoma. Antiserum to gamma-irradiated control (acetone-treated) cells was negative. Thus gamma-irradiation of carcinogen-treated cells before injection failed to abolish specific antibody responses in immunized rats. To investigate further the relationships between cell-carcinogen interaction, neoantigen induction and malignancy, the cells of a non-immunogenic, spontaneous mammary carcinoma were treated with MCA in vitro, and antisera against treated and untreated cells were tested against a panel of established tumours. A positive membrane-immunofluorescence reaction was obtained with an antiserum to MCA-treated cells, but not to untreated cells against an aminoazodye-induced hepatoma, indicating that the previously non-immunogenic mammary carcinoma cells had acquired new antigenic specificities as a consequence of carcinogen treatment.
先前的研究表明，体外经3-甲基胆蒽（MCA）处理的大鼠胚胎细胞能在同基因大鼠中引发抗体，这些抗体能特异性地与已建立的MCA诱导的肉瘤发生反应。为了检验一个或几个抗原性的、癌前克隆的克隆扩增是否是先前观察到的特异性抗体反应的原因，在将MCA处理的大鼠胚胎细胞注射到宿主动物体内之前，先对其进行了150戈瑞的γ射线照射。通过膜免疫荧光和同位素抗球蛋白试验，筛选所得抗血清对一组已建立的同基因肿瘤的反应性。在针对γ射线照射的MCA处理细胞产生的抗血清池与一种免疫原性自发乳腺癌细胞之间观察到阳性反应。针对γ射线照射的对照（丙酮处理）细胞的抗血清为阴性。因此，注射前对致癌物处理的细胞进行γ射线照射未能消除免疫大鼠中的特异性抗体反应。为了进一步研究细胞-致癌物相互作用、新抗原诱导与恶性肿瘤之间的关系，体外用MCA处理一种非免疫原性的自发乳腺癌细胞，并用针对处理和未处理细胞的抗血清检测一组已建立的肿瘤。针对MCA处理细胞的抗血清对氨基偶氮染料诱导的肝癌呈现阳性膜免疫荧光反应，而针对未处理细胞的抗血清则无此反应，这表明先前非免疫原性的乳腺癌细胞因致癌物处理而获得了新的抗原特异性。
