Dilakian E A
Biull Eksp Biol Med. 1981 Mar;91(3):319-21.
The presence of porcine kidney cortex of the SH-dependent enzymes, lysosomal carboxypeptidase B and cathepsins B, H and L, was demonstrated. A method for isolation of these enzymes was described which involves gel filtration through Sephadex G-100, covalent chromatography on mercury-organic Sepharose 4B and rechromatography on Sephadex G-100. Specific activity of lysosomal carboxypeptidase B was increased 9000-fold by BAA splitting and that of cathepsin B 240-fold by BAPA splitting. The molecular weights of lysosomal carboxypeptidase B and cathepsin B were found to be 50,000 and 26,000, respectively. Both enzymes were identical with appropriate enzymes obtained from other sources and differed in their properties from high-molecular-weight SH-proteinase or porcine kidney cortex.
已证实猪肾皮质中存在依赖巯基的酶，即溶酶体羧肽酶B以及组织蛋白酶B、H和L。描述了一种分离这些酶的方法，该方法包括通过葡聚糖凝胶G - 100进行凝胶过滤、在汞 - 有机琼脂糖凝胶4B上进行共价层析以及在葡聚糖凝胶G - 100上进行再层析。通过BAA裂解，溶酶体羧肽酶B的比活性提高了9000倍，通过BAPA裂解，组织蛋白酶B的比活性提高了240倍。发现溶酶体羧肽酶B和组织蛋白酶B的分子量分别为50,000和26,000。这两种酶与从其他来源获得的相应酶相同，并且在性质上与高分子量巯基蛋白酶或猪肾皮质不同。
